Tennessee and Florida will play in Gainesville as scheduled on Saturday at 3:30 p.m.
The Southeastern Conference made the announcement late Tuesday morning after speculation about how Hurricane Irma might affect the game.
"The University of Florida has been diligent in assessing the impact of Hurricane Irma on the Gainesville community and has made a thoughtful decision in moving forward with the game," SEC Commissioner Greg Sankey said in a statement released by the league.
The Gators (0-1) canceled their home opener against Northern Colorado this past weekend in anticipation of the storm.
Tennessee (2-0) has not won in Gainesville since 2003. The Volunteers snapped an 11-game losing streak to the Gators with a victory in Knoxville last season.
"We appreciate the communication from both our friends in Gainesville and the conference office as this assessment was made," Tennessee athletic director John Currie said in the league statement. "Our thoughts and prayers are with those affected by Hurricane Irma and other recent storms throughout the SEC footprint, and we're grateful for the opportunity to travel to Florida this weekend."